Fluorescent derivatization of a protease antigen to track antigen uptake and processing in human cell lines

BMC Immunology

Table 5 Tracking FITC-Subtilisin in a large scale antigen uptake experiment with immuno-affinity purification^a

Fraction	RFUs	Protein (μg)	% Recovered
Pulsed Cells	133800	223000	100%
Washed Cells	1358	22630	1
Cell lysate	558	930	0.4
Immunoaffinity column flow-through	343	571	0.20
< 10 K flow-through fraction	79.2	132	0.06
DR-eluted peptides	31	51	0.02

^a8.5 × 10⁹ 8.1.6 cells were mixed with 223 mg of conjugate (0.6 RFU/ug) in 1600 ml of complete media and incubated at 37°C for 22 hr.

ISSN: 1471-2172