Figure 2

Enzymatic activity and western blot analysis of cynomolgus CD38. A. Ecto-cyclase activity was evaluated by measuring conversion of NGD to fluorescent cGDPR by NIH/mac38 (1), NIH/hum38 (2), NIH/3T3 (3), or cynomolgus macaque RBCs (4) and human RBCs (5). Each bar represents mean ± SD; (*) means significantly higher than controls (P < 0.05, t-test, n = 3 experiments). Y axis = fluorescence emission 410 nm. B. Western blot of lysates from parental NIH/3T3 cells (NIH), NIH/hum38 (hum38) and NIH/mac38 cells (mac38) analysed by 10% SDS-PAGE under non-reducing (NR) conditions. Blots were probed with AT1 anti-human CD38 mAb. C. Western blot of parental NIH/3T3 and NIH/mac38 cell lysates analysed by 8% SDS-PAGE in non-reducing (NR) or reducing (R) conditions and probed with the indicated anti-cynomolgus CD38 mAbs. In B and C, molecular weight markers (in kDa) are indicated on the right.