PCR efficiency of cytokines and related molecules among rat organs and structures. Slope values are related to PCR efficiencies (Ex) using the equation Ex = (10-1/slope) - 1. The mean efficiency is 0.965 ± 0.085. The red line matches PCR efficiency of 1 (slope = -3.32). Slope values are assessed using LightCycler 3 software (ROCHE), from templates coming from two brain structures (hypothalamus, hippocampus) and five organs (ileum, liver, lung, spleen, skin). RNA was isolated using phenol RNA extraction. The primer sets are listed in Tables 1, 2 and amplification conditions are described in "methods". Each value is the mean of fourteen independent experiments. Data are means ± SEM.