Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green

Table 1 Primer sequences used for cytokine and cytokine receptor real-time PCR assays

Gene name		5'-3' primer sequence	Position cDNA-gene^a	Accession Number^b
IL1a	FW	AAGACAAGCCTGTGTTGCTGAAGG	663–747	D00403
	RW	TCCCAGAAGAAAATGAGGTCGGTC	Int. span.	NW_047658
IL1b	FW	CACCTCTCAAGCAGAGCACAG	793–871	M98820
	RW	GGGTTCCATGGTGAAGTCAAC	Exon	NW_047658
IL1RA	FW	AAGACCTTCTACCTGAGGAACAACC	139–255	M63101
	RW	GCCCAAGAACACATTCCGAAAGTC	Int. span.	NW_047651
IL1R1	FW	GTTTTTGGAACACCCTTCAGCC	1209–1313	M95578
	RW	ACGAAGCAGATGAACGGATAGC	Exon	NW_047814.1
IL1R2	FW	CATTCAGACACCTCCAGCAGTTC	328–443	Z22812
	RW	ACCCAGAGCGTATCATCCTTCAC	Exon	NW_047814.1
IL6	FW	TCCTACCCCAACTTCCAATGCTC	532–610	E02522
	RW	TTGGATGGTCTTGGTCCTTAGCC	Exon	M26745
IL6R	FW	AAGCAGGTCCAGCCACAATGTAG	696–812	NM_017020
	RW	CCAACTGACTTTGAGCCAACGAG	Int. span.	NW_047626.1
TNFa	FW	AAATGGGCTCCCTCTCATCAGTTC	195–305	X66539
	RW	TCTGCTTGGTGGTTTGCTACGAC	Int. span.	D00475
TNF-r2	FW	TGCAACAAGACTTCAGACACCGTG	142–224	AF420214
	RW	AGGCATGTATGCAGATGGTTCCAG	Exon	NW_047727.1

Note. FW forward primer; RW reverse primer; IL, interleukin; IL-r, interleukin receptor; TNFα, Tumor necrosis factor α; TNF-r2, Tumor necrosis factor receptor 2. ^a Position of amplification product within cDNA sequence (upper line) and within genomic sequences (lower line). Exon; both primers bound on the same exon, Int. span.: primers bound on different exons, , ^b Genbank accession number of cDNA (upper line) and genomic sequences or contigs (lower line), available at http://www.ncbi.nlm.nih.gov/

ISSN: 1471-2172