Figure 6

β-gal⁺ EC trigger immune responses and are cleared from TIE2-lacZ skin grafts onto wild type FVB but not VWF-lacZ hosts. A. ELISA of sera from reciprocal donor-host skin transplants detects β-gal specific antibodies in FVB but not TIE2-lacZ hosts. Similar results were obtained in 3 separate experiments, in which 7 out of 8 wild type mice generated strong IgG2a isotype antibodies to β-gal following TIE2-lacZ skin grafts. B. VWF-lacZ mice do not raise β-gal-specific antisera following grafting with TIE2-lacZ skin. Grafting and antisera were analyzed as described above.C. EC expressing β-gal are cleared from TIE2-lacZ skin grafts onto host wild type FVB but not VWF-lacZ mice. Photomicrographs of the underside of X-gal stained donor TIE2-lacZ skin and TIE2-lacZ skin grafts from FVB or VWF-lacZ hosts are shown.