Figure 5

Competition assays of various chemokines on U-937/rabbit CCR2 stable transfectants. (A) U-937/rabbit CCR2 stable cells (104 cells/well) were incubated with ¹²⁵I-mouse JE (0.11 nM) for 2 hours in the presence of unlabeled chemokines (Human MCP-1, -2, -3, -4) at concentrations as high as 30 nM. (B). U-937/rabbit CCR2 stable cells (10⁴ cells/well) were incubated with ¹²⁵I-mouse JE (0.11 nM) for 2 hours in the presence of unlabeled chemokines (mouse JE, hMIP-1α, hMIP-1β and hRANTES) at concentrations as high as 30 nM. (C) Competition binding assay was performed on U-937/rabbit CCR2 stable cells (10⁴ cells/well) that were incubated with ¹²⁵I-mouse JE (0.11 nM) for 2 hours in the presence of various concentrations of TAK-779. Each point represents mean values ± standard deviations of the duplicates from one of two representative assays.