Table 2 Summary of assay characteristics
From: Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT
Assay | Type of assay | Readout | Fresh to frozen correlation | Sensitivity and specificity1 | Inter-assay correlations |
|---|---|---|---|---|---|
Tetramer | Phenotypic | 4-color flow cytometry | r = 0.9 | >70%2 | r = 0.9 |
CFC | Functional | 4-color flow cytometry | r = 0.6 (CMV) r = 0.4 (SEB) | >70%2 | r = 0.9 r = 0.7 |
ELISPOT | Functional | Plate reader | r = 0.6 (CMV) NS (SEB) | >90% | r = 0.7 |