Figure 4

A Mannose receptor-fusion protein recognises distinct bands in blots of salivary gland and pancreas tissue lysates. Tissue lysates were separated by SDS-PAGE and examined by Coomassie protein staining and western and lectin blotting as indicated. The doublet of bands recognised by the antibody directed against the putative MR ligand, salivary amylase, did not correspond to the bands recognised by the MR-fusion protein, CRD4-7Fc. The specificity of this probe was confirmed by loss of all bands in blots probed with CRD4-7Fc in the presence of the competitor, mannose. Binding to mannose-BSA is shown as a positive control. Protein loaded per lane was 10 μg of salivary gland and pancreas lysates in each application except western blotting of pancreas in which 200 ng was used. 5 ng mannose-BSA was used per lane in lectin blotting. The migration pattern of molecular weight markers is indicated on the left of the image.