CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use

Table 1 Primer sequences used for Cyproquant assays Table shows position of amplification product within cDNA sequence (upper line) and within genomic sequence (lower line). GenBank accession numbers are indicated.

Gene name	5'-3' primer sequence		Position cDNA-gene	Accesion Number
IL-1β	FW	CCTGTCCTGCGTGTTGAAAGA	639–788	NM_000576
	RW	GGGAACTGGGCAGACTCAAA	Int. Span.	M15840
IL-4	FW	AACAGCCTCACAGAGCAGAAGAC	177–278	BC070123
	RW	GCCCTGCAGAAGGTTTCCTT	Int. Span.	AF395008
IL-10	FW	GCTGGAGGACTTTAAGGGTTACCT	210–318	AY029171
	RW	CTTGATGTCTGGGTCTTGGTTCT	Int. Span.	AF418271
IL-12p40	FW	CATGGTGGATGCCGTTCA	636–767	AF180563
	RW	ACCTCCACCTGCCGAGAAT	Int. Span.	AY008847
IL-13	FW	ACAGCCCTCAGGGAGCTCAT	135–231	NM_002188
	RW	TCAGGTTGATGCTCCATACCAT	Int. Span.	AF377331
IL-15	FW	CCATCCAGTGCTACTTGTGTTTACTT	351–466	U14407
	RW	CCAGTTGGCTTCTGTTTTAGGAA	Int. Span.	X91233
IL-18	FW	GACGCATGCCCTCAATCC	966–1071	NM_001562
	RW	CTAGAGCGCAATGGTGCAATC	Exon	E17138
IFN-γ	FW	GTTTTGGGTTCTCTTGGCTGTTA	151–263	NM_000619
	RW	AAAAGAGTTCCATTATCCGCTACATC	Int. Span.	AF375790
MIF	FW	GCCCGGACAGGGTCTACA	322–399	M25639
	RW	CTTAGGCGAAGGTGGAGTTGTT	Int. Span.	L19686
TGF-β1	FW	CAAGGGCTACCATGCCAACT	1786–1869	NM_000660
	RW	AGGGCCAGGACCTTGCTG	Int. Span.	X05839
TNF-α	FW	CCCCAGGGACCTCTCTCTAATC	358–455	NM_000594
	RW	GGTTTGCTACAACATGGGCTACA	Int. Span.	AB088112
β2-MG	FW	AATTGAAAAAGTGGAGCATTCAGA	255–389	NM_004048
	RW	GGCTGTGACAAAGTCACATGGTT	Exon	M17987
UBC	FW	ATTTGGGTCGCGGTTCTTG	20–152	BC039193
	RW	TGCCTTGACATTCTCGATGGT	Int. Span.	D63791
YWHAZ	FW	ACTTTTGGTACATTGTGGCTTCAA	1090–1183	NM_003406
	RW	CCGCCAGGACAAACCAGTAT	Exon	NC_000008

Note: FW = Forward primer; RW = Reverse primer; Int. Span. = Intron spanning, i.e. Primers derived from different exons; Exon = both primers derived from the same exon. β2-MG = β2-microglobulin. UBC = Ubiquitin C. YWHAZ = tyrosine 3-monooxygenase tryptophan 5-monooxygenase activation protein, zeta polypeptide.

ISSN: 1471-2172