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Table 4 RNA and DNA standard-based quantification of TNF-a, IL-1β and IFN-γ transcripts Fifty million copies of TNF-α, IL-1β and IFN-γ standard RNA were amplified in parallel of a range of RNA standard (CyProQuant-PCR) and DNA standard. Copy numbers were directly deduced from the cycle at threshold. *For TNF-α, the experiment was repeated at two days interval.

From: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use

Target gene Actual copy number Calculated copy number
   CyProQuant-PCR DNA standard approach
TNF-α* 5 107 5.2 107 7.7 105
  5 107 5.0 107 3.6 105
IL-1β 5 107 4.3 107 6.7 105
IFN-γ 5 107 5.5 107 7.8 105