Table 4 RNA and DNA standard-based quantification of TNF-a, IL-1β and IFN-γ transcripts Fifty million copies of TNF-α, IL-1β and IFN-γ standard RNA were amplified in parallel of a range of RNA standard (CyProQuant-PCR) and DNA standard. Copy numbers were directly deduced from the cycle at threshold. *For TNF-α, the experiment was repeated at two days interval.
Target gene | Actual copy number | Calculated copy number | |
|---|---|---|---|
CyProQuant-PCR | DNA standard approach | ||
TNF-α* | 5 107 | 5.2 107 | 7.7 105 |
5 107 | 5.0 107 | 3.6 105 | |
IL-1β | 5 107 | 4.3 107 | 6.7 105 |
IFN-γ | 5 107 | 5.5 107 | 7.8 105 |