Figure 1

An overview of the polypeptides encoded by SH2D2A transcript variants. A. Schematic presentation of putative protein-interacting domains in the SH2D2A isoforms and mutants described in this study: The SH2D2A-1 encodes the full length TSAd, whereas the SH2D2A-2 and -3 variants represent different N-terminal sequences of TSAd. The SH2D2A-4 variant has a 10 aa insertion in the SH2-domain, whereas the fifth variant, SH2D2A-5, lacks aa239–334, containing the proline rich region including the four C-terminal tyrosines (Y = tyrosine). TSAd 4YF has the four C-terminal tyrosines exchanged with phenylalanine (F = phenylalanine). TSAd d239–256 has a deletion of amino acids 239–256 containing the motif PSQLLRPKPPIPAKPQLP. B. Comparison of the N-terminal amino acid sequences of the SH2D2A-1, -2 and -3 variants: The amino acid position is numbered according to full length TSAd (SH2D2A-1). The SH2D2A-2 and -3 variants differ from the SH2D2A-1 in the N-terminal 1–21 aa and 1–28 aa, respectively. C. Comparison of the N-terminus SH2 domain aa sequences of the SH2D2A-1 and -4 variants: The conserved arginine (R) at position 120 is marked. The SH2D2A-4 has an additional 10 aa (ins aa103–112) in the TSAd-SH2 domain. A SH2 consensus (con) sequence obtained from a Blast search, [42] is included for comparison. Conserved residues are marked in bold.