Figure 6
From: Enumeration of leukocyte infiltration in solid tumors by confocal laser scanning microscopy
Illustration of image thresholding. To separate antigenic staining from background and noise, a threshold is applied to remove grey scales below a desired level (in this case 8). The graph shows the fluorescence intensity profile of the complete image slice (derived from an image stack). A threshold (dotted red line) is placed to separate data from non-fluorescent background and additional noise. All pixels (voxels in the complete stack) are then used for subsequent calculations.