Figure 2
Influence of Pro-Th1 (IL-2, IFN-γ) and Pro-Th2 (IL-1, IL-4, IL-5) cytokines on the proliferation and IL-4 and IL-5 secretion by Th2 cells when B cells, splenic macrophages and peritoneal macrophages were used as APC. D10G4.1 Th2 cells were cultured with B cells, splenic and peritoneal macrophages and conalbumin (100 μg/ml). Cytokines IL-1, IL-2, IL-4 or IL-5 and IFN-γ were also added in the cultures. Cultures with peritoneal macrophages also received 1 mM aminoguanidine. For proliferation, the cultures were pulsed after 48 h with 3H-thymidine (0.5 μCi/well) and harvested after last 16 h of incubation period. Data are expressed as mean ± SEM of triplicate wells (Fig. 2a). For IL-4 (Fig. 2b) and IL-5 (Fig. 2c), the supernatants were collected from the triplicate wells after 48 h of the initiation of cultures, pooled and estimated by ELISA. The data are expressed as pg/ml. The control cultures comprising Th2 cells, Th2 cell+Ag, APCs+Ag, APCs+Th2 cells showed no apparent change. The data presented are from three independent experiments. '*' Represents p < 0.001 and '◆' represents p < 0.05. SMQ and PMQ represent splenic and peritoneal macrophages respectively.