Figure 5
Influence of Pro-Th1 (IL-2 and IL-12) and Pro-Th2 (IL-4) cytokines on the proliferation and IFN-γ secretion by Th1 cells stimulated with anti-CD3 Ab and B7-1. pGL-10 Th1 cells were cultured with anti-CD3 Ab (0.01 μg/ml) in the presence of CHO-B7-1 (5 × 104/well). Cytokines IL-2, IL-4 or IL-12 alone or in combinations were also added into the cultures. For proliferation (Fig. 5a), cultures were pulsed after 48 h with 3H-thymidine (0.5 μCi/well) and were harvested after last 16 h of incubation period. Data are expressed as mean ± SEM of triplicate wells. IFN-γ (Fig. 5b) was estimated by ELISA in the pooled culture supernatants collected from the triplicate wells after 48 h of the initiation of cultures. The control cultures comprising of Th1 cells, CHO-B7-1+Th1 cells, CHO+Th1 cells, CHO-B7-1, CHO showed no noticeable change. The data presented are from three independent experiments. '*' Represents p < 0.001.