Figure 1
From: Optimized detection of circulating anti-nuclear envelope autoantibodies by immunofluorescence
(A) Immunostaining of HeLa cells fixed with 1% or 4% formaldehyde with PBC sera diluted 1/80, Bar = 20 μm. (B) Immunobloting depicting electrophoretically fractionated nuclei and nuclear envelopes (NEs) from HeLa cells that have been probed with serum 27 (s27), serum 159 (s159), serum 161 (s161), serum 184 (s184), serum 176 (s176) or serum 30 (s30) at a dilution 1/300. At the left molecular mass markers at 125, 103, 49, 33, 23 and 18 kDa are indicated.