Figure 3From: Optimized detection of circulating anti-nuclear envelope autoantibodies by immunofluorescenceImmunostaining of HeLa cells with PBC sera. Normally growing HeLa cells fixed with methanol, ethanol, 1% or 4% formaldehyde were stained with sera (s) 27, 28, 152, 159 and 161 diluted 1/80, Bar = 20 μm.Back to article page