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Table 1 Comparison of PBMC cytokine secretion using central versus peripheral laboratory study models

From: Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study

  IL-5a IL-13a TNFa IFN-γa IL-10a IL-8b IL-12a
PHA        
- Peripheral lab (fresh) 26 (8.7) 422* (1.9) 4,400* (3.0) 73,000* (1.4) 557* (0.9#)   
- Central lab (cryopreserved) 19 (8.0) 252* (3.3) 2,100* (2.0) 15,000* (4.0) 38* (7.1#)   
LPS        
- Peripheral lab (fresh)    2,600* (1.3) 68 (10) 981* (2.7) 332* (1.9) 7.9 (9.9#)
- Central lab (cryopreserved)    1,600* (5.2) 117(15) 708* (3.1) 141* (1.9) 6.6 (24#)
TT        
- Peripheral lab (fresh) 8.2 (6.0) 555 (5.3) 493* (13) 81,400* (8.8) 24* (3.1#)   
- Central lab (cryopreserved) 7.2 (26) 714 (2.5) 103* (1.4) 10,900* (1.6) 7* (20#)   
  1. Values are geometric mean (apg/ml, bng/ml); Percent coefficient of variation shown in parentheses. Red color and * indicates <0.01 for difference in cytokine produced between fresh v. cryopreserved PBMC; Blue color and # indicates <0.05 for difference in variance