Skip to main content

Table 4 Comparison of LPS response in U937 cells, THP-1 and PBMC macrophages.

From: Transcriptional profiling of the LPS induced NF-κB response in macrophages

      PBMC macrophage
  U937 THP-1 Donor A Donor B
  Time of LPS treatment
Gene 1 4 1 4 1 4 1 4
Chemokines/Receptors
CCL3 18 19 (± 3) 1622 (± 322) 141 (± 27) 22 57 90 89
CCL3L1 ND 21 (± 7) 56 (± 3) 4 (± 1) 62 51 271 83
CCL4 30 (± 11) 228 (± 63) 7047 (± 836) 2481 (± 714) 31 154 197 445
CCL8 ND ND 8 (± 2) 94 (± 38) 11 134 42 175
CCL22 NC NC NC 135 (± 28) NC 3 NC NC
CXCL1 ND ND 258 (± 41) 250 (± 21) 74 86 308 91
CXCL2 * 15 (± 3) 8 (± 3) 243 (± 85) 68 (± 13) 97 35 260 16
CXCL8 7 (± 1) 9 (± 3) 1016 (± 57) 491 (± 56) 118 280 282 178
CXCR4 NC NC NC -4 (± 1) 2 NC 2 -4
Cytokines/Receptors
IL-1β 5 6 (± 1) 3490 (± 254) 1891 (± 135) 139 277 905 325
IL-1RN NC NC 4 6 (± 2) 11 56 21 75
PBEF NC 2 5 (± 2) 19 (± 1) 2 24 2 37
TNF-α* 9 (± 2) 3 392 (± 55) 39 (± 4) 164 144 626 233
Stress
COX-2 14 (± 4) 17 (± 5) 166 (± 34) 29 (± 8) 291 888 432 333
SOD2 * 2 7 44 (± 7) 264 (± 37) 6 42 7 31
TXNIP NC NC -11 (± 2) -2 (± 1) -3 -2 -3 -3
Apoptosis
cIAP2 * 6 (± 1) 12 19 (± 3) 112 (± 11) 11 54 16 26
GADD45β NC NC 7 (± 1) 8 (± 1) 4 18 10 24
PDCD4 NC NC NC -7 (± 1) NC -2 NC -3
PMAIP1 NC NC 7 (± 1) NC 127 74 213 34
TNFAIP3 * 17 (± 1) 15 (± 3) 104 (± 17) 44 (± 8) 54 63 83 39
Transcription
C-MYB NC NC NC -5 (± 1) NC NC -2 3
TOP2α NC NC NC -3 NC -3 NC -2
C-REL NC NC 4 (± 1) 38 (± 3) 2 15 3 8
IκB-α* 8 (± 2) 6 40 (± 4) 19 (± 2) 18 17 20 16
NFκB1 NC 2 4 (± 1) 36 (± 2) 3 35 4 27
Cell cycle/Differentiation
BTG2 4 (± 1) 5 (± 2) 116 (± 21) 32 (± 7) 5 3 8 NC
BUB1β NC NC NC -3 NC -5 NC -4
ID2 NC NC NC 4 (± 1) NC 2 NC 2
JARID1B NC NC NC NC NC 2 NC NC
Growth
IGFBP3 ND ND 43 (± 9) 1420 (± 167) ND 12 ND 46
Adhesion
ICAM1 * 2 5 (± 1) 6 70 (± 44) 7 19 10 20
Miscellaneous
HLA-DRα 2 (± 4) NC NC NC NC 2 NC 2
SMYD5 NC NC NC NC NC -2 NC -2
  1. Real time PCR results correspond to mean fold change normalised to GAPDH from 3 biological replicates of U937 cells treated with LPS and SEM is indicated. Genes showing an identical expression profile between THP-1 cells, U937 cells and PBMC derived macrophages from both donors are indicated with an *