Table 4 Comparison of LPS response in U937 cells, THP-1 and PBMC macrophages.
From: Transcriptional profiling of the LPS induced NF-κB response in macrophages
PBMC macrophage | ||||||||
|---|---|---|---|---|---|---|---|---|
U937 | THP-1 | Donor A | Donor B | |||||
Time of LPS treatment | ||||||||
Gene | 1 | 4 | 1 | 4 | 1 | 4 | 1 | 4 |
Chemokines/Receptors | ||||||||
CCL3 | 18 | 19 (± 3) | 1622 (± 322) | 141 (± 27) | 22 | 57 | 90 | 89 |
CCL3L1 | ND | 21 (± 7) | 56 (± 3) | 4 (± 1) | 62 | 51 | 271 | 83 |
CCL4 | 30 (± 11) | 228 (± 63) | 7047 (± 836) | 2481 (± 714) | 31 | 154 | 197 | 445 |
CCL8 | ND | ND | 8 (± 2) | 94 (± 38) | 11 | 134 | 42 | 175 |
CCL22 | NC | NC | NC | 135 (± 28) | NC | 3 | NC | NC |
CXCL1 | ND | ND | 258 (± 41) | 250 (± 21) | 74 | 86 | 308 | 91 |
CXCL2 * | 15 (± 3) | 8 (± 3) | 243 (± 85) | 68 (± 13) | 97 | 35 | 260 | 16 |
CXCL8 | 7 (± 1) | 9 (± 3) | 1016 (± 57) | 491 (± 56) | 118 | 280 | 282 | 178 |
CXCR4 | NC | NC | NC | -4 (± 1) | 2 | NC | 2 | -4 |
Cytokines/Receptors | ||||||||
IL-1β | 5 | 6 (± 1) | 3490 (± 254) | 1891 (± 135) | 139 | 277 | 905 | 325 |
IL-1RN | NC | NC | 4 | 6 (± 2) | 11 | 56 | 21 | 75 |
PBEF | NC | 2 | 5 (± 2) | 19 (± 1) | 2 | 24 | 2 | 37 |
TNF-α* | 9 (± 2) | 3 | 392 (± 55) | 39 (± 4) | 164 | 144 | 626 | 233 |
Stress | ||||||||
COX-2 | 14 (± 4) | 17 (± 5) | 166 (± 34) | 29 (± 8) | 291 | 888 | 432 | 333 |
SOD2 * | 2 | 7 | 44 (± 7) | 264 (± 37) | 6 | 42 | 7 | 31 |
TXNIP | NC | NC | -11 (± 2) | -2 (± 1) | -3 | -2 | -3 | -3 |
Apoptosis | ||||||||
cIAP2 * | 6 (± 1) | 12 | 19 (± 3) | 112 (± 11) | 11 | 54 | 16 | 26 |
GADD45β | NC | NC | 7 (± 1) | 8 (± 1) | 4 | 18 | 10 | 24 |
PDCD4 | NC | NC | NC | -7 (± 1) | NC | -2 | NC | -3 |
PMAIP1 | NC | NC | 7 (± 1) | NC | 127 | 74 | 213 | 34 |
TNFAIP3 * | 17 (± 1) | 15 (± 3) | 104 (± 17) | 44 (± 8) | 54 | 63 | 83 | 39 |
Transcription | ||||||||
C-MYB | NC | NC | NC | -5 (± 1) | NC | NC | -2 | 3 |
TOP2α | NC | NC | NC | -3 | NC | -3 | NC | -2 |
C-REL | NC | NC | 4 (± 1) | 38 (± 3) | 2 | 15 | 3 | 8 |
IκB-α* | 8 (± 2) | 6 | 40 (± 4) | 19 (± 2) | 18 | 17 | 20 | 16 |
NFκB1 | NC | 2 | 4 (± 1) | 36 (± 2) | 3 | 35 | 4 | 27 |
Cell cycle/Differentiation | ||||||||
BTG2 | 4 (± 1) | 5 (± 2) | 116 (± 21) | 32 (± 7) | 5 | 3 | 8 | NC |
BUB1β | NC | NC | NC | -3 | NC | -5 | NC | -4 |
ID2 | NC | NC | NC | 4 (± 1) | NC | 2 | NC | 2 |
JARID1B | NC | NC | NC | NC | NC | 2 | NC | NC |
Growth | ||||||||
IGFBP3 | ND | ND | 43 (± 9) | 1420 (± 167) | ND | 12 | ND | 46 |
Adhesion | ||||||||
ICAM1 * | 2 | 5 (± 1) | 6 | 70 (± 44) | 7 | 19 | 10 | 20 |
Miscellaneous | ||||||||
HLA-DRα | 2 (± 4) | NC | NC | NC | NC | 2 | NC | 2 |
SMYD5 | NC | NC | NC | NC | NC | -2 | NC | -2 |