Therapeutic MIF infusion severely abrogates the course of EAE. (A) No statistically significant difference was observed in EAE onset between wt PBS and wt MIFd7 mice. However, the symptom severity was dramatically decreased (p = 0.0005) in wt MIFd7 compared to wt PBS; recovery was complete (p = 0.0005). wt PBS n = 18; wt MIF d7 n = 11. (B) Luxol Fast Blue staining shows very little demyelination at day 23 in wt MIFd7 mice. The dashed line and the asterisk demarcate the ventral column of the spinal cord. The intensity of luxol fast blue staining within the ventral column was quantified using the NIH Image freeware and was normalized to day 0 staining. Background staining was subtracted. (C) Immunohistochemistry for reactive macrophages/microglia using antibody against F4/80. (D) Immunohistochemistry for CD3+ T cells.