Prophylactic administration of Tuftsin in wild-type mice results in a dampened disease course. (A) Disease onset in wt tufd-1 mice does not differ from wt PBS mice. Severity is dramatically decreased (p = 0.008) and recovery is complete (p = 0.016). wt PBS n= 18, wt tufd-1 n = 13. (B) Luxol fast blue histological stain reveals levels of myelination. The dashed line and the asterisk demarcate the ventral column of the spinal cord. The intensity of luxol fast blue staining within the ventral column was quantified using the NIH Image freeware and was normalized to day 0 staining. Background staining was subtracted. (C) Reactive macrophages/microglia are visible in the coronal sections of experimental mice at different timepoints. (D) Infiltrating T cells were detected by immunohistochemistry using an anti-CD3 antibody.