Figure 4

Therapeutic use of Tuftsin in wt mice results in a severely abrogated course of disease. (A) Disease onset is the same in wt tufd7 mice and wt PBS mice. Severity is strongly dampened (p = 0.004) and recovery is complete (p = 0.0002). wt PBS n= 18, wt tufd7 n = 13. (B) Levels of myelination are visualized using luxol fast blue. The dashed line and the asterisk demarcate the ventral column of the spinal cord. The intensity of luxol fast blue staining within the ventral column was quantified using the NIH Image freeware and was normalized to day 0 staining. Background staining was subtracted. (C) Macrophage/microglial activation is visualized with F4/80 staining. (D) Immunohistochemistry for infiltrating T cells using an anti-CD3 antibody.