Figure 2

Macrophage fusion and cell-to-cell Cn transfer involves actin. 3D reconstructed confocal images show infected macrophages fusing (A). Cutting along the Z-axis of panel (A) demonstrates that the intracellular Cn are being shared by both macrophages and are surrounded by a high concentration of phalloidin-labeled actin from both macrophages, as indicated by the arrow (B). Panel (C) shows an uninfected macrophage. Images were collected at 63×.