Figure 3
From: Haematopoietic development and immunological function in the absence of cathepsin D
Flow cytometry of major T cell populations in CCDWT and CCDD thymus. Thymocytes were collected from chimaeras at least three months post-reconstitution, or a control age matched Balb/c mouse and stained for various lineage markers as described in Materials and Methods. a) Forward/side scatter plot for thymus cells showing normal distribution of size/granularity in both CCDWT and CCDD. Gate shows region analysed for plots b. b) Single and double staining with anti-CD4 and anti-CD8 antibodies. c) Single colour staining for T cells with anti-CD3 antibody. One representative staining of at least 5 separate experiments on different mice of each chimaera type is shown. The % of cells staining for each type is shown in each quadrant.