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Figure 3 | BMC Immunology

Figure 3

From: Mycobacterium tuberculosis 6-kDa Early Secreted Antigenic Target (ESAT-6) protein downregulates Lipopolysaccharide induced c-myc expression by modulating the Extracellular Signal Regulated Kinases 1/2

Figure 3

Stimulation with ESAT-6 in presence of sodium orthovanadate caused appearance of phospho-ERK1/2 in the nucleus. Stimulation of RAW264.7 cells with 5 μg/ml of ESAT-6 and 1 mM Na3VO4 for 0, 15, 30, 60 and 120 minutes. (A) Phosphorylation of ERK1/2 in cytoplasm. (C) ERK1/2 phosphorylation in the nucleus. (B) and (D) Total ERK1/2 in cytoplasm and nucleus respectively. (E) and (G) Phosphorylation of ERK1/2 in cytoplasm and the nucleus respectively upon treatment with 1 mM Na3VO4 for 0, 15, 30, 60 and 120 minutes. (F) and (H) Total ERK1/2 in cytoplasm and nucleus respectively to show equal loading of proteins in all the lanes. The data is representative of three independent experiments.

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