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Figure 7 | BMC Immunology

Figure 7

From: Mycobacterium tuberculosis 6-kDa Early Secreted Antigenic Target (ESAT-6) protein downregulates Lipopolysaccharide induced c-myc expression by modulating the Extracellular Signal Regulated Kinases 1/2

Figure 7

ESAT-6 downregulated LPS induced c-myc expression. (A) RT-PCR for c-myc gene expression, Lane. 1. Unstimulated cells, Lane.2. Cells stimulated with 5 μg/ml ESAT-6, Lane.3. Cells stimulated with 0.1 μg/ml of LPS, Lane. 4. Cells stimulated with LPS and ESAT-6, Lane.5. Cells stimulated with 1 mM Na3VO4 and 5 μg/ml ESAT-6, Lane.6. Cells stimulated with 1 mM Na3VO4. Stimulation time was 120 minutes. (C) RT-PCR for c-myc gene expression, Lane.1. Unstimulated cells, Lane.2. Cells stimulated with 5 μg/ml ESAT-6, Lane.3. Cells stimulated with 1 mM Na3VO4 and 5 μg/ml ESAT-6, Lane.4. Cells stimulated with Na3VO4 and ESAT-6 and 10 μM of MEK-1 inhibitor PD98059, Lane.5. Cells simulated with Na3VO4 and ESAT-6 and 10 μM of p38 inhibitor SB203580, Lane.6. Cells stimulated with 1 mM Na3VO4, (B) and (D) RT-PCR for β-actin to confirm equal amplification in all the samples. The data is representative of two independent experiments. (E) and (F) shows the graph of densitometric analysis of Figure. 8A and 8C respectively. The data is shown as fold change; unstimulated cells were given a value of 1.00.

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