Figure 4
From: Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo
Neutrophil adhesion and spreading (mean ± SD) on immobilized fibrinogen. Adhesion and spreading of isolated Syk-/- (gray bar) or control neutrophils (black bar) with or without (w/o) addition of 1 mM Mn2+ at 37°C for 30 min. Adherent Syk-/- (n = 7 mice) or control neutrophils (n = 5 mice) in percent of total cells added (A), microscopic images (B), increase of cell area (in μm2, C) and frequency distribution of cell area (D) of adherent Syk-/- (n = 400 from 4 mice) and control neutrophils (n = 400 from 4 mice) upon stimulation for 30 min at 37°C. * indicates significant difference (p < 0.05), n.s., not significant.