Figure 1

Gene Expression of DEK and other Neuroblastoma Tumor-Associated Antigens Identified by Immune Serum-SEREX. Expression of antigens in mouse neuroblastoma cell lines (the parental line for our vaccine, Neuro-2a, and TBJ) and normal mouse tissues are presented as a heatmap. A Total 22 of 25 SEREX identified genes listed in Table 1 are present in the mouse cDNA microarray chip and were used for the hierarchical clustering analysis. In the case of multiple clones representing the same gene, the average of expression ratios was used (labeled as GeneName_avg). The Pearson correlation matrix was used for clustering. Expression level of each gene was log2 transformed and z-score normalization was performed across samples for each gene. The expression was represented by pseudo-colors according to the scale shown on the bottom. A red color corresponds to up-regulation and a green color corresponds to down-regulation compared with the mean.