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Figure 9 | BMC Immunology

Figure 9

From: Coexpression of GM-CSF and antigen in DNA prime-adenoviral vector boost immunization enhances polyfunctional CD8+ T cell responses, whereas expression of GM-CSF antigen fusion protein induces autoimmunity

Figure 9

GM-CSF autoantibodies do not interfere with the control of FV infection. GM-CSF autoantibodies were induced by DNA prime adenoviral vector boost immunization with the GM-OVA vaccines. As controls, groups of mice were immunized with the OVA or the GM-DP-OVA vaccines, or were mock vaccinated (PBS). Two weeks after the adenoviral vector booster immunization, mice were challenged with 3000 SFFU of FV. 11 days post challenge FV-specific immune responses were monitored by MHC I (A) and MHC II (C) tetramer staining. Mean percentages and SEM (n = 6) of Tet+ CD8+ (A) and CD4+ (C) cells are shown. Splenocytes of an uninfected animal were used as negative control (naiv). The viral load in the blood was detected by AB34+ cells via flow cytometry (B). In addition the numbers of infectious centers per spleen were determined (D).

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BMC Immunology

ISSN: 1471-2172

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