Figure 3

Predicted DRB1*0402-specific immunological hot spots for Dsg1 and Dsg3 glycoproteins. Multiple sequence alignment of Dsg1 and Dsg3. Predicted hot spots (threshold = -123 kJ/mol) shaded in black, experimental Dsg3 hot spots highlighted red, and experimentally confirmed DRB1*0402-specific Dsg3 peptides highlighted blue. Dsg1 – Signal Peptide, SP: 1–23; Propeptide, PP: 24–49; Extracellular domain 1, ECD1: 50–158; ECD2: 159–270; ECD3: 271–385; ECD4: 386–497; ECD5: 498–548; Transmembrane, TM: 549–569; Intracellular domain, ICD: 570–1049. Dsg3 – SP: 1–23; PP: 24–49; ECD1: 50–158; ECD2: 159–268; ECD3: 269–383; ECD4: 386–499; ECD5: 500–615; TM: 616–640; ICD: 641–999.