Figure 1From: Molecular cloning and expression analysis of the STAT1 gene from olive flounder, Paralichthys olivaceusCloning of flounder STAT1 cDNA. (A) Comparison of the domain structure of human STAT1 to zebrafish STAT1. ND, N-domain responsible for dimer-dimer interactions; COILED-COIL, coiled-coil domain responsible for protein-protein interactions; DNA, DNA-binding domain; SH2, src homology 2 domain responsible for receptor binding and dimerization. (B) The degenerative PCR primers used in the experiments. (C) PCR strategies used to clone flounder STAT1. The STAT5'-1 and STAT3' primers were used for first-round PCR, and the STAT5'-2 and STAT3' primers were used for nested PCR.Back to article page