Figure 2

Antimicrobial peptide expression along the mouse small intestine. A) CRS, cryptdins, pLys, and sPLA2 mRNA expression in small intestine measured by qRT-PCR. Absolute mRNA copy numbers were determined in the duodenum, jejunum, and ileum. The "x" numbers denote relative copy numbers between ileum and duodenum. The results are based on four FVB mice, and are given as mean and range. B) Comparative distributions in duodenum and ileum. Of note is that duodenum has a total of 2 × 10⁶ copies of Paneth cell products, while ileum has 18 × 10⁶ copies (area of the smaller duodenal and the ileal pies are proportional to total mRNA copy counts of analyzed antimicrobials). Colours denote individual Paneth cell products. C) Western blot of extracts from two individual BALB/c mice was analyzed using antibodies to CRS4C-3 and cryptdin 2. Duod = duodenum, Jej = jenunum and Ile = ileum.