Figure 1

Peritoneal MΦs from db/db mice and MΦs cultured in diabetic conditions have decreased macropinocytosis. A, peritoneal MΦs were isolated from non-diabetic (db/+) and diabetic (db/db) and FITC-albumin uptake was measured by flow cytometry.B, MΦs were cultured in high glucose/high insulin conditions for 24 h or 48 h. FITC-albumin uptake was measured by flow cytometry. C, MΦs were cultured for 48 h in high glucose/high insulin conditions and FITC-albumin uptake was visualized by fluorescent microscopy. Results are representative of at least three independent experiments ± SEM. *: p < 0.05 from low glucose controls.