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Figure 4 | BMC Immunology

Figure 4

From: CD11c+ antigen presenting cells from the alveolar space, lung parenchyma and spleen differ in their phenotype and capabilities to activate naïve and antigen-primed T cells

Figure 4

Antigen-specific transgenic T cell activation by peptide-pulsed CD11c+ APCs from BAL, lung parenchyma and spleen. CD11c+ APC populations isolated from BAL, lung and spleen were pulsed with OVA OT-I (SIINFEKL) or OT-II peptide and co-cultured for 48 hours with CFSE-labelled OT-I CD8+ T cells or for 72 hours with CFSE-labelled OT-II CD4+ T cells. Transgenic T cell proliferation was analyzed by FACS. A, OT-I CD8 T cell proliferation. B, OT-II CD4 T cell proliferation. Representative histograms of T cell proliferation are shown. The average T cell proliferation rates are shown in bar graphs after subtracting from appropriate controls. Results are presented as mean ± SD of triplicate samples. *p < 0.05.

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BMC Immunology

ISSN: 1471-2172

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