Table 3 Primer sequences used to identify gene transcript levels by real-time PCR
From: Intragraft gene expression profile associated with the induction of tolerance
Gene Symbol | Â | Sequence (5' -> 3') |
|---|---|---|
lenti-porcine GalT | sense | GTT CGC TTC TCG CTT CTG TT |
| Â | antisense | CCA AAA CAC AAC CAT TAC AGT TGA G |
Ptger4 (EP4) | sense | TAC TTC TAC AGC CAC TAC GTG GAC |
| Â | antisense | TGG TCC AGT CGA TGA AGC ACC AGG |
Tnfsf10 (Trail) | sense | ACC ACG TGC TCT TTA GGA ATG GAG |
| Â | antisense | AGA CCA TCT TGG AAG CGT CTT CAG |
Il2rg (CD132) | sense | GGT TGG AAC GAA TGC CTC CAA TTC |
| Â | antisense | GCA GAA CCG TTC ACT GTA GTC TGG |
Stat3 | sense | GCA AAG AGT CAC ATG CCA CGT TGG |
| Â | antisense | AGA TAC CTG CTC TGC AGA AAC TGC |
Cfh | sense | AAG GTG GCA GTC ATT ACC TCG CTG |
| Â | antisense | GTT CAT GAC TGC TGG ACT CAA TGG |
Cxcl12 | sense | CGC TCT GCA TCA GTG ACG GTA AAC |
| Â | antisense | CTT CAG CCG TGC AAC AAT CTG AAG |
Hfe | sense | TCT CTA AGG TGT CAG GCT CTG GAC |
| Â | antisense | TGT CAG CCA GCC TTG ATA GGT CTC |
Foxp3 | sense | TCC AGA GAG AAG TGG TGC AGT CTC |
| Â | antisense | GTG GCT ACG ATG CAG CAA GAG CTC |
Hmox1 (HO-1) | sense | ACA GAT GGC GTC ACT TCG TCA GAG |
| Â | antisense | ACT GCC ACT GTT GCC AAC AGG AAG |
Bcl2 | sense | GAT GCC TTT GTG GAA CTA TAT GGC |
| Â | antisense | AGG TAT GCA CCC AGA GTG ATG CAG |
Tnfaip3 (A20) | sense | CTA AGC CAA CGA GTA GGT TCT GTG |
| Â | antisense | CCA TAC ATC TGC TTG AAC TGG TAG |
GAPDH | sense | GGC ATG GAC TGT GGT CAT GAG |
| Â | antisense | TGC ACC ACC AAC TGC TTA GCC |