Figure 4

Suppressive activity exhibited by CD8⁺CD94⁺T cells from ACAID mice in vitro. A: the purity of isolated CD8⁺CD94⁺T (left) and CD8⁺CD94^-T (right) cells by magnetic affinity cell sorting; B: the response of CD8⁺CD94⁺T and CD8⁺CD94^-T cells to anti-CD3 mAb stimulation (stim). *p < 0.05 compared with unstimulated group. C: suppressive effect of CD8⁺CD94⁺T and CD8⁺CD94^-T cells on the proliferation of splenic MNC. Splenic MNC (5 × 10⁴ cells/well, as responder cells) from immunized mice were cocultured with purified CD8⁺CD94⁺T or CD8⁺CD94^-T cells (as regulatory cells) from ACAID mice and immunized mice at different responder/suppressor ratios (1:0, 1:0.25, 1:0.5 and 1:1) in the presence of OVA(100 μg/ml) and anti-CD3 mAb(1 μg/ml) for 72 hours and pulsed with [3H]thymidine for the last 16 hours of culture. Data are represented as mean ± SEM of triplicate samples. *p < 0.05 compared with stimulated MNC group. D: suppressive effect of CD8⁺CD94⁺T and CD8⁺CD94^-T cells from ACAID mice on the proliferation of splenic MNC from naive mice (at 1:1 ratio) upon anti-CD3 mAb(1 μg/ml) stimulation.