Figure 1From: Direct contact of platelets and their released products exert different effects on human dendritic cell maturationPlatelet binding to dendritic cells. Platelet (PLT) binding to dendritic cells (DCs) was evaluated by flow cytometry (A-D) or by confocal microscopy (E-G). DCs and PLTs were cultured for 48 h alone (A-B) or together, either in two 0.4-μm pore-sized filter-separated compartments (C) or in the same compartment (D). DCs and PLTs were stained for CD1a and CD41, respectively, and analyzed by flow cytometry (A-D). For PLTs cultured alone, flow cytometry plots are not gated on any marker. For DCs, flow cytometry plots are gated on CD1a+ events only. Plots are representative of five independent experiments. (E-G) DCs cultured with PLTs in the same compartment were stained for CD209 and CD41 and analyzed by confocal microscopy (E, anti-CD41-FITC; F, anti-CD209- Alexa Fluor® 555 and G, anti-CD41-FITC, and anti-CD209- Alexa Fluor® 555). The confocal microscopy photograph is representative of three independent experiments.Back to article page