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Figure 1 | BMC Immunology

Figure 1

From: Transglutaminase activity in the hematopoietic tissue of a crustacean, Pacifastacus leniusculus, importance in hemocyte homeostasis

Figure 1

TGase is an abundant protein in Hpt cells. A. Hpt cell lysate separated by 12.5% SDS-PAGE. The main protein band around 90 kDa was confirmed to be the crayfish TGase by LC-MS. The strong band at 50 kDa (arrow) was confirmed to be actin. B. TGase transcript levels in Hpt cells and in separated circulating SG and G hemocytes analyzed by RT-PCR. Control transcript is 40S ribosomal protein. Lane 1, Hpt cell TGase; lane 2, SG-cell TGase; lane 3, G-cell TGase; lane 4, Hpt cells 40S; lane 5, SG-cell 40S; lane 6, G-cell 40S; M is 100 bp DNA marker. C. TGase activity in Hpt cell lysate and in lysate from separated SG or G hemocytes. The amount of incorporated 5' (biotinamido) pentylamine was quantified by measuring the absorbance at 450 nm in a plate reader. Mean ± SD of 3 separate experiments.

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BMC Immunology

ISSN: 1471-2172

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