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Table 1 Mean response levels of the three CMV-responsive donors

From: Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides

  % CD3+CD8+ cells ELISPOT (peptide mix) CFC (peptide mix) CFC (pp65495–503) Tetramer (pp65495–503) ELISPOT (pp65495–503)
Donor 41 (low) 20.3% 270 SFC1 [0.53%] nd2 0.06%2 0.06%2 8 SFC1 [0.02%]
Donor 68 (med) 12.1% 285 SFC1 [0.94%] 0.28%2 0.28%2 0.25%2 31 SFC1 [0.10%]
Donor 43 (high) 26.3% 95 SFC1 [0.14%] 0.87%2 0.93%2 1.27%2 28 SFC1 [0.04%]
  1. 1 ELISPOT results reported as SFC per 2.5 × 105 PBMC. Numbers in brackets indicate the values when re-calculated as % of CD8+ T cells. Note that ELISPOT responses with peptide mix did not match the other assays in terms of low, medium, and high responders.
  2. 2 CFC and tetramer results reported as % of CD8+ T cells. nd = CFC was not done on this donor due to predicted very low response (0.02%) seen in pre-screening.