Figure 3
From: Limited role for ASC and NLRP3 during in vivo Salmonella Typhimurium infection
Severe liver pathology in a typhoid mouse model is ASC and NLRP3 independent. Representative slides of liver haemotoxylin and eosin (HE) staining of WT (A, B), Asc−/−(C, D) and Nlrp3−/−(E, F) mice 2 (A-C) and 5 (D-F) days after infection with S. Typhimurium (106; typhoid model). All groups showed marked inflammation. Arrowheads indicate inflammatory cell influx. Original magnification, × 10. Total pathology score (G, H) of the liver determined 2 and 5 days post infection in WT (white), Asc−/− (grey) and Nlrp3−/− (black) according to the scoring system as described in Methods. Median values (straight lines) are shown. Graphs depict 8–12 mice per genotype and per time-point. NB. Experiments at day 5 were performed with all three genotypes simultaneously; for reasons of clarity WT results are used in both Asc- and Nlrp3 graphs.