Figure 2
From: T lymphocytes maintain structure and function of fibroblastic reticular cells via lymphotoxin (LT)-B
Down-regulation of the spleen chemokines after T cell absence and altered cell localization after down-regulation of lymphoid chemokine. Spleen cryostat sections from BALB/c mice and nude mice were stained for CCL21 (red, Scale bars: 200um), (A), RT-qPCR of chemokine expression in whole spleen of BALB/c and nude mice (B), chemokine expression in the spleen of BALB/c and nude mice were quantified by ELISA in tissue homogenates (C), Naive T lymphocytes were purified and labeled with CFSE and transferred into mice, and their localization in the spleen white pulps was ascertained 8 hours later (Scale bars: 50um) (D). Quantitation of CFSE + cells in the spleen white pulps after transfer,The number of CFSE + cells present in WP regions is shown (E), Data are representative of 5 mice per group.