Skip to main content
Figure 1 | BMC Immunology

Figure 1

From: Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in electrophoretic mobility shift assays

Figure 1

Efficient release of nuclei from cells using hypo-osmotic buffer and pipetting. Phase-contrast microscopic image of THP-1 cells in Lysis Buffer before (A) and after (B) pipetting through a 200-μl pipette tip. Original magnification: ×200. Arrowheads indicate membrane components around the nuclei that were observed before (A), but not after (B), pipetting.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us