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Figure 2 | BMC Immunology

Figure 2

From: Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in electrophoretic mobility shift assays

Figure 2

Nuclear protein extracts contain active NF-κB heterodimers. Non-adherent monocytic THP-1 cells were stimulated by TPA at the indicated concentrations for 30 minutes. Nuclear proteins were subsequently purified from THP-1 cells and EMSA was performed using an NF-κB-specific DNA probe (lanes 1–3). For specific competition experiments, unlabeled excess (125-fold molar) probe was pre-added to the proteins (lane 4).

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BMC Immunology

ISSN: 1471-2172

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