Figure 5
From: Adoptive T-cell therapy of prostate cancer targeting the cancer stem cell antigen EpCAM
Human PBLs transduced with EpCAM-specific CARs kill PC3 cells. PBLs transduced with the EpCAM-specific CAR or control vector were incubated with PC3-luc cells at E:T ratios of 1:1 or 2:1. Luciferin was added 24 h later and cell viability was determined by luminescence imaging. PC3-luc cells incubated alone were used as controls. Statistical analysis was performed using a Student’s t-test.