Phenotypic characteristics of IFN-α-DCs in normal pregnant women and pregnant women with hyperandrogenia. IFN-α-DCs were analyzed by flow cytometry for the expression of DC-related surface antigens in non-pregnant reproductive aged women (control group), normal pregnant women (NP-women), and pregnant women with hyperandrogenia (HA-women). (A) Histograms represent the expression of the indicated molecules (bold-line histograms) and the corresponding isotype controls (grey-filled histograms) in individual experiments. The relative percentage of positive cells (%) among the DCs are presented as means (± SE). (B) Gated HLA-DR+ IFN-α-DCs were analyzed for CD14 and CD83 staining. Representative stainings of IFN-α-DCs from NP-women (left, dot plots) and HA-women (right, dot plots) are shown.