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Table 3 IFN-α-DC induced apoptosis of CD3 + CD4 + and CD3 + CD8 + Т cells

From: Elevated levels of dehydroepiandrosterone as a potential mechanism of dendritic cell impairment during pregnancy

Annexin V + /PI (%) Untreated MNCs, n = 8 MNCs + DCs (NP), n = 6 MNCs + DCs (HA), n = 8
CD3+CD4+ 0.19 ± 0.05 4.7 ± 1.0* 5.3 ± 1.2*
Т cells 0.25 3.6 3.92
CD3+CD8+ 0.17 ± 0.04 15.02 ± 0.3* 11.19 ± 0.9*#
Т cells 0.13 15.0 11.23
  1. Untreated MNCs from healthy donors (control) and MNCs co-cultured with IFNα-DCs from normal pregnant (NP) women or pregnant women with hyperandrogenia (HA) at a MNC-to-DC ratio of 10:1 for 3 days were analyzed for Annexin V/PI double positivity by flow cytometry. Data are presented as mean (± SE) percentages and medians of apoptotic Annexin V+/PI cells within the CD3+CD4+ or CD3+CD8+ (CD4) gates. *, pU <0.05, compared to the control group; #, pU < 0.01, a significant difference between the NP pregnant women and HA pregnant women groups; differences were detected using the Mann–Whitney U test.