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Table 3 IFN-α-DC induced apoptosis of CD3 + CD4 + and CD3 + CD8 + Т cells

From: Elevated levels of dehydroepiandrosterone as a potential mechanism of dendritic cell impairment during pregnancy

Annexin V + /PI − (%)

Untreated MNCs, n = 8

MNCs + DCs (NP), n = 6

MNCs + DCs (HA), n = 8

CD3+CD4+

0.19 ± 0.05

4.7 ± 1.0*

5.3 ± 1.2*

Т cells

0.25

3.6

3.92

CD3+CD8+

0.17 ± 0.04

15.02 ± 0.3*

11.19 ± 0.9*#

Т cells

0.13

15.0

11.23

  1. Untreated MNCs from healthy donors (control) and MNCs co-cultured with IFNα-DCs from normal pregnant (NP) women or pregnant women with hyperandrogenia (HA) at a MNC-to-DC ratio of 10:1 for 3 days were analyzed for Annexin V/PI double positivity by flow cytometry. Data are presented as mean (± SE) percentages and medians of apoptotic Annexin V+/PI− cells within the CD3+CD4+ or CD3+CD8+ (CD4−) gates. *, pU <0.05, compared to the control group; #, pU < 0.01, a significant difference between the NP pregnant women and HA pregnant women groups; differences were detected using the Mann–Whitney U test.
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BMC Immunology

ISSN: 1471-2172

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