Figure 2

Flow cytometry analysis of CD63 expression on CD41 ⁺ platelets. Platelets were or stimulated or not with TRAP (50 μg/ml, 30 min at RT). Platelets from platelet-rich plasma (PRP; black bars), washed (PBS) platelet suspensions (khaki bars) or washed (PBS) platelet suspensions with added recombinant soluble CD14 (white bars) were tested. Platelets were then stimulated, or not, with E. coli LPS (1–10 μg/ml; 30 min at RT), stained with allophycocyanin-conjugated anti-CD41 and phycoerythrin-conjugated anti-CD63 mAbs. Gating was made on the platelet population, (R5 region according to FSC/SSC comparable to the Figure 1 cytogram). Double positive cells (CD41 + CD63+) were then measured. Data are expressed as fold increase of controls (± SD; n = 10 independent experiments). *P < 0.05 (Wilcoxon paired test; stimulated vs. non-stimulated).