Skip to main content
Figure 3 | BMC Immunology

Figure 3

From: C-reactive protein enhances activation of coagulation system and inflammatory response through dissociating into monomeric form in antineutrophil cytoplasmic antibody-associated vasculitis

Figure 3

Western-bolt with 1/20 of standard SDS. A: Western-Blot assay of the CRP deposited on platelets. A typical result from 3 experiments was shown. pCRP control ran as a smear without a single band (lane 1), while mCRP control ran as a single band with a molecular weight of 15 kDa (lane 2). The substances deposited on slides ran as a 15 kDa single band without a smear after perfusion experiment with pCRP (60 μg/mL)-containing PRP (lane 3). A stronger band was detected after perfusion experiment with mCRP (60 μg/mL)-containing PRP (lane 4). The relative intensity of lane 4/lane3 was 2.45 ± 0.63. No smear or single band was detected after perfusion experiment with PRP which did not contain pCRP or mCRP (lane 5). B: Western-Blot assay of the co-incubation of isolated NETs and pCRP. A typical result from 3 experiments was shown. Lane 1: pCRP control. Lane 2: mCRP control. Lane 3: a smear without a single band was detected after pCRP was statically incubated with isolated NETs for 12 hr.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us