Figure 3

Percentage of Th17 cells in blood, spleen and draining lymph nodes. (A) At 2wks postoperative, the level of Th17 cells in recipients with a single administration of either ATRA or TGF-β was significantly lower than those treated with a control solution. A mixed treatment of these two agents could further suppress Th17 cells in lymph nodes at 2wks and 8 wks. Data are presented as the mean value ± standard error and are representative of at least three independent experiments. (B) Representative flow cytometric figures of IL-17 and CD8 expression on CD3 + CD4+ cells from the blood, spleen and LN in recipient mice. * P < 0.05, ** P < 0.01, ***P < 0.001.