Serum reactome induced by Bordetella pertussis infection and Pertussis vaccines: qualitative differences in serum antibody recognition patterns revealed by peptide microarray analysis

Table 3 Sequence of target epitopes exclusively recognized in serum from individuals either with the natural B. pertussis infection or after DTPa2 vaccination

*B. pertussis* protein	GENE	B-cell epitope
*Children with whooping cough*
Pertussis toxin subunit 4 precursor	PtxS4	CFGKDLKRPGSSPME
P.69A protein (pertactin)	Prn	LWYAESNALSKRLGE
P.69A protein (pertactin)	Prn	AVVHLQLATIRRGDA
Filamentous hemagglutinin	fhaB	FAADLRTVYAKQADQ
Bifunctional hemolysin-adenylate cyclaseprecursor	cyaA	HAANQAVDQAGIEKL
Outer membrane porin protein precursor	ompP	FGVNTFADGFKANSY
*Children who received the the DTPa2 vaccine*
Bifunctional hemolysin-adenylate cyclaseprecursor	cyaA	DQTVSGLEIGLDRGV
Tracheal colonization factor	tcfA	ASNGLRIKDDGTNSM

The Table shows the sequence of target epitopes specifically recognized in serum (10/10) children with whooping cough (n = 6 peptides) and in serum from children who receivedthe Diphtheria, Tetanus, Pertussis acellular 2 components (DTPa2) vaccine (2 peptides). Peptide microarray slides containing unique Bp peptides (n = 3,085 peptides) were processed and analyzed. We list here only peptides which were exclusively recognized in serum samples from individuals either with the natural Bp infection or after DTPa2 vaccination and never recognized in any serum samples from individuals who received the placebo (i.e. DT ).

ISSN: 1471-2172