Figure 1

Screening of E-FABP inhibitors. T cells were isolated and incubated with potential E-FABP inhibitors (10 μM) or DMSO control for overnight. Expression of PPARβ/δ (A) and PPARγ (B) was examined by realtime-PCR (*, p < 0.05 as compared to the DMSO control). (C) Confocal analysis of PPARγ protein expression in BMDCs cultured either with DMSO or with EI-03 (10 μM) for 18 hours (green: PPARγ, blue: nuclei). (D) Chemical structure of the EI-03 (2-(4-acetylphenoxy)-9,10-dimethoxy-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one). (E) Analysis of EI-03/E-FABP complex by a computational model. The protein was shown as a ribbon diagram and a partially transparent surface representation while the docked compound is shown as a ball-and-stick model. The inset shows a zoomed-in view of the main interactions highlighted by a network of hydrogen bonds.